ISSN 2500-2236
DOI-prefix: 10.18527/2500-2236

First report of the fungus Diaporthe phaseolorum on sunflower in Russia


received: 2018-08-21    accepted: 2018-10-25    published: 2018-11-23   

M. M. Gomzhina#, Ph. B. Gannibal

Author Affiliations

All-Russian Institute of Plant Protection, St. Petersburg, Russian Federation
# Corresponding author: Maria Gomzhina, e-mail: gomzhina91@mail.ru


Abstract

Fungi in the ascomycete genus Diaporthe have been identified worldwide. Typically, the Diaporthe species are saprobes, endophytes, or plant pathogens. The distinction between the species of this genus has historically been based on an approach that combined morphological information, cultural characteristics, and host affiliation. The correct identification of the Diaporthe species should be continued based on a combination of molecular, morphological, cultural, phytopathological, and mating type data. A comprehensive analysis of the Diaporthe species in Russia has never been conducted. 

Therefore, the biodiversity and geographical distribution of Diaporthe spp. in Russian Federation must be updated. The goal of this study was the identification of the isolate Diaporthe sp. MF 16-010 extracted from the stems of Helianthus annuus collected in the Krasnodar region of Russian Federation. 

According to the morphology data and DNA sequence analyses of the nuclear ribosomal internal transcribed spacer (ITS) region and genes of the translation elongation factor-1α (EF-1α) and ß-tubulin, the isolate MF 16-010 was identified as Diaporthe phaseolorum (Cooke & Ellis) Sacc. To the best of our knowledge, this isolate represents the first report of Diaporthe phaseolorum associated with sunflower in Russian Federation. We showed that the artificial inoculation to plants has resulted in the development of stem lesions, which allows us to conclude that isolate MF 16-010 is pathogenic for sunflower.


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